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The several steps of T. whipplei cell culture are summarized in Fig. Fig.1. 1.Culture was performed by the centrifugation-shell vial technique with a human fibroblast cell line (HEL). These cells are routinely used, and more than 6,000 attempts to culture intracellular organisms have been performed in our laboratory using this cell line (unpublished data).
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Sea Shell Crusher from Sunco Machinery can be used to crush the sea shell into smaller size. After being crushed by the sea shell crusher, the crushed sea shell can be grinded by the sea shell mill (Raymond Mill) into fine powder.After being grinded by the raymond mill, the powder output size can be from 30 mesh to 325 mesh.Finally the sea shell powder can be used to produce animal feed.
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Nov 05, 2009· A Hybodotid shark crusher teeth called Ptychodus whipplei. These crusher teeth were used eat shell fish. The fossil was found in the Upper Cretaceous Period (about 70 million years ago). It was found in Dallas County, Texas in the Kamp Ranch Formation. Thanks to Herb for the images.
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Jennifer Viegas, 'Shell Crusher' Shark Swam Cretaceous Kansas. Discovery News (23 februari 2010). Geraadpleegd op 23 februari 2010. Mike Everhart, The Ptychodontid Sharks. Oceans Of Kansas Paleontology (2008). Geraadpleegd op 23 februari 2010.
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The survival and replication of T. whipplei using the centrifugation shell-vial technique was the first step in enhancing the understanding of this fastidious bacterium.
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The first illustration of a Ptychodus tooth () was published by Morton (1834) but was not further identified than "The palate bones of a Fish?"The specimen was later sent to Dr. Mantell in England for identification. The tooth was later figured again and identified by Morton (1842), and noted to have been found by "Mr. Conrad in the older cretaceous strata at Prairie Bluff, Alabama."
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The culture of Tropheryma whipplei, the bacterium responsible for Whipple's disease, has been established only recently. Our objective is to describe, based on our experience, the culture of T. whipplei in HEL cells detected by immunofluorescence staining. Over 3 years, we received 18 samples for T. whipplei culture from 15 patients with Whipple's disease.
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